LHX3 Interacts with Inhibitor of Histone Acetyltransferase Complex Subunits LANP and TAF-1β to Modulate Pituitary Gene Regulation
Figure 2
Mapping of LHX3-INHAT interactions using radiolabeled deletion constructs.
(A) LHX3-LANP interaction was mapped using radiolabeled deletion constructs. The indicated in vitro translated 35S-labeled LANP expression constructs were incubated with either GST alone (control) or GST-LHX3 resins. Resins were washed at least four times in interaction buffer and associated proteins separated by SDS-PAGE. Gels were fixed and incubated in Amplify fluorography reagent (Amersham). Dried gels were exposed to Biomax MR film (Kodak). Input = 10% of input radiolabeled LANP lysate. (B) Mapping of LHX3-TAF-1β interactions using radiolabeled deletion constructs. The indicated in vitro translated 35S-labeled TAF-1β expression constructs were incubated with either GST alone (control) or GST-LHX3 resins. Reactions were washed at least four times in interaction buffer and separated by SDS-PAGE. Gels were fixed and incubated in Amplify fluorography reagent (Amersham). Dried gels were exposed to Biomax MR film (Kodak). Input = 10% of radiolabeled TAF-1β lysate.