Temperature Increase Exacerbates Apoptotic Neuronal Death in Chemically-Induced Ischemia
Figure 1
Temperature increase exacerbated sodium azide/2-DG-induced cell death.
(A) Sodium azide caused dose-dependent cell death. Neuronal death was induced with different concentrations of sodium azide plus 0.5 mM 2-DG for a period of 1 hour. The time point of cell collection for the analysis was decided based on the findings from our preliminary studies. Cell survival was detected with trypan blue exclusion assay. Error bars represent mean ± SEM. n = 4. * denotes significant difference from 37°C control cell (0 mM) (p<0.01). (B) Increased temperature resulted in more cell death. Cells were treated as indicated for a period of 1 hour and cell survival was detected with trypan blue exclusion assay. * denotes significant difference from 37°C control (p<0.01). † denotes significant difference from 37°C with sodium azide/2-DG (p<0.01). ‡ denotes significant difference from 41°C without sodium azide/2-DG (p<0.05).