Inhibition of GSK 3β Activity Is Associated with Excessive EZH2 Expression and Enhanced Tumour Invasion in Nasopharyngeal Carcinoma
Figure 7
GSK3β-enhanced migration and invasion of NPC cells were abrogated by EZH2 siRNA transfection.
(A–C) The siRNA knockdown efficiency was evaluated by testing the protein level of EZH2. EZH2 siRNA transfection significantly reduced EZH2 expression in CNE-1 and CNE-2 cells; (D,E) Inhibition of EZH2 by EZH2 siRNA (50 nmol/L) transfection significantly inhibited migration of CNE-1 and CNE-2; (F,G) EZH2 siRNA (50 nmol/L) transfection significantly inhibited GSK3β-KD-dependent migration of CNE-1 and CNE-2; (H,I) Inhibition of EZH2 by EZH2 siRNA (50 nmol/L) transfection significantly inhibited invasion of CNE-1 and CNE-2; (J,K) EZH2 siRNA transfection significantly inhibited GSK3β-KD-dependent invasion of CNE-1 and CNE-2. Migration and invasion of NPC cells were evaluated after EZH2 siRNA (50 nmol/L) and/or GSK3β-KD plasmid (2 μg/mL) were transfected for 48 or 72 h. The data indicate the means (SEM) of 3 independent experiments. NC: normal control; KD: kinase-dead GSK-3β plasmid.