Sip1, an AP-1 Accessory Protein in Fission Yeast, Is Required for Localization of Rho3 GTPase
Figure 1
Isolation of Rho3 as a multicopy suppressor of sip1-i4 mutant cells.
(A) sip1-i4 cells were transformed with either the pDB248 multicopy vector, vector containing sip1+ or vector containing rho3+. Cells were then streaked onto plates containing 0.5 µg/mL FK506, 0.5 µg/mL micafungin, 0.3 M MgCl2, or 6 mM valproic acid and incubated at 27°C for 4 d or at 36°C for 3 d, respectively. (B) sip1-i4 cells transformed with the multicopy vector pDB248, or the genome DNA clones containing rho1+, rho2+, rho3+, rho4+, rho5+, or cdc42+ were streaked onto plates containing 0.5 µg/mL FK506, 0.3 M MgCl2, or 6 mM valproic acid then incubated at 27°C for 4 d or at 36°C for 3 d, respectively.