Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot
Figure 4
Cell cycle analysis of human KG1a leukemic cell line exposed to colcemid.
The cells were stained with 7-AAD and antibodies anti-Ki67 and anti-phospho(Ser10)-histone H3 conjugated to Alexa Fluor®488. An isotype control staining with Alexa Fluor®488 mouse IgG1 was performed. Effects of colcemid (0.1 µg/mL, 30 min and 1 h, 37°C, 5% CO2). The results present the percentage of cells in the apoptosis and all cell cycle phases. (representative experiment).