Identification of Novel Stress Granule Components That Are Involved in Nuclear Transport
Figure 10
Interaction of purified importin-α1 with RNA homopolymers in vitro.
His6-importin-α1 or His6-poly(A)-binding protein was synthesized in E. coli. Purified importin-α1 was incubated with immobilized poly(A), poly(U), poly(C) or poly(G) homopolymers or non-conjugated resin. In control experiments, resins were pre-treated with micrococcal nuclease as indicated. Aliquots of the flow through (10%, Ft) and bound material (100%, B) were analyzed by Western blotting with antibodies against importin-α1 (top). Under the same conditions, a strong interaction between immobilized poly(A) and purified poly(A)-binding protein was detected with antibodies against the His-tag (bottom).