Identification of Novel Stress Granule Components That Are Involved in Nuclear Transport
Figure 9
The association of importin-α1 with poly(A)-RNA in growing cells is regulated by stress.
Crude extracts prepared for controls (ethanol, EtOH) and DEM-treated cells were incubated with oligo-(dT)-cellulose as described in Materials and methods. Aliquots of the start (St, 10%) and eluted (E, 100%) material were analyzed by Western blotting with different antibodies as indicated. The relative binding was calculated for importin-α1 and HuR; results were normalized to control samples. Data for three independent experiments are shown as average +SEM. Student’s t-test was applied to identify significant differences between control and DEM-treated samples; *, p<0.05. There was no significant change for HuR.