Identification of Novel Stress Granule Components That Are Involved in Nuclear Transport
Figure 7
Quantification of the SG association for importin-α1, α4, α5 and importin-β1.
HeLa cells were incubated with DEM, arsenite or heat shock and stained with antibodies against importin-α1, α4, α5, or importin-β1. SGs were identified with the marker protein HuR, and 30 SG-containing cells were evaluated for every treatment. Each individual SG was scored for the presence of importin-α1, α4, α5 or importin-β1. A single data point represents the average of three independent experiments+SEM. The tables depict the number of SGs that were positive for the importin analyzed/number of SGs identified with HuR; results are shown for each individual experiment. Note that more than 90% of SGs were positive for the examined members of the importin-α family or importin-β1.