Evidence for Glutamate as a Neuroglial Transmitter within Sensory Ganglia
Figure 8
Representative images from a trigeminal ganglion immunostained for glutamate following CCI of the ION.
Increased glutamate immunolabeling is seen in the trigeminal ganglion ipsilateral to the CCI (A, standard, C, thresholded image) compared to the contralateral side (B, standard, D, thresholded image). I, shows the % difference in glutamate immuno-expression between the ipsi and contralateral trigeminal ganglion for sham, 4, 7 and 14 days post CCI. Ganglia from sham-operated rats (E, standard, G, thresholded image) showed no difference compared to contralateral ganglia (F, standard, H, thresholded image). J, white bars show total glutamate immunopositive cells sorted by size from 9 sections of three 14-day CCI-ION trigeminal ganglion and the black bars show the number glutamate positive cells above threshold. Scale bar = 50 µm *, P<0.05; **, P<0.01; ***, P<0.001.