Evidence for Glutamate as a Neuroglial Transmitter within Sensory Ganglia
Figure 3
Representative calcium imaging fluorescent traces of a neuron and SGC.
A, B, following application of 200 µM glutamate both neurons and SGCs showed an increased fluorescent ratio during the 10 minutes of recording. A1, B1 after washing with HEPES buffer, KCl (50 mM) was given to identify neurons in the recorded field. Ionomycin (20 µM) was added to test for cell viability at the end of the experiment. Lines show the duration of application for experimental agent. Scale bar = 5 minutes. C, average relative glutamate induced maximum change of 340/380 fluorescence ratio from pre-drug (baseline, normalized at 0) condition. Paired Student’s t- tests were used to compare pre-drug and post-drug conditions. Mean ± S.E.M. ***P<0.001.