Evidence for Glutamate as a Neuroglial Transmitter within Sensory Ganglia
Figure 2
Confocal and calcium influx images of a 24-hour DRG primary mixed culture.
A–C confocal image showing SGCs (red: glutamine synthetase an SGC marker, open arrow), neuron (green: β-tubulin, solid arrow) and nucleus (blue: DAPI). D–F representative pictures from a 24-hour culture used for calcium imaging. D and E (high magnification) were taken before the start of calcium imaging. F, fluorescent image following 200 µM glutamate application showing an increase in the 340/380 ratio indicated by an increase in signal intensity in the neuron (N), and two SGCs (yellow arrows) compared to the SGC which did not respond (white arrow). C, D, E, F, scale bar: 10 µm.