Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids
Figure 6
Growth phenotype of mtlD inactivated S. aureus.
(A) Culture of strains in broth containing Mtl at 37°C. Liv1023 (SH1000 mtlD::tet) (•) had a significantly reduced growth rate (P<0.01, Student’s t-test) compared to wild-type (SH1000) (▪), Liv1024 (SH1000 mtlABFD::tet) (▴), strains Liv1098 (SH1000 mtlD::tet pMJH71) (□) and Liv1097 (SH1000 mtlABFD::tet pMJH71) (○). Calculated doubling times between 2 h and 3 h of growth: SH1000 = 0.33, Liv1023 (SH1000 mtlD::tet) = 1.7. Error bars indicate 1 SEM (n = 3). (B) Culture of strains in BHI broth at 25°C. Liv1023 (SH1000 mtlD::tet) (•) has a significantly reduced growth rate (P<0.001, Student’s t-test) compared to wild-type (SH1000) (▪), Liv1024 (SH1000 mtlABFD::tet) (▴), strains Liv1098 (SH1000 mtlD::tet pMJH71) (□) and Liv1097 (SH1000 mtlABFD::tet pMJH71) (○). Calculated doubling times between 5 h and 13 h of growth: SH1000 = 2.46, Liv1023 = 3.13. Representative dataset from triplicate assay.