Structural Insights into Clostridium perfringens Delta Toxin Pore Formation
Figure 5
HeLa cells were incubated with 5 µg/ml Cy3-Delta toxin for 30min at 4°C (lane 1), 5 µg/ml for 30 min at 37°C (lane 2), or 10 µg/ml for 30 min at 37°C (lane 3) in DMEM medium containing 0.1% BSA.
After washing, the cell lysates were elctrophoresed in a SDS-containing 10% polyacrylamide gel without reducing agent and scanned for fluorescence.