Cellular FRET-Biosensors to Detect Membrane Targeting Inhibitors of N-Myristoylated Proteins
Figure 2
NANOMS report on chemical inhibition of NMT.
(A) FRET-responses of Yes-, Src- and Gi2-NANOMS transfected BHK cells treated with 4 µM of the specific NMT inhibitor DDD85646. The error bars denote the s.e.m (n = 5). Samples were statistically compared with the untreated control. See Methods for more information on statistical analysis. (B) Confocal sensitized acceptor FRET-imaging of Yes-NANOMS expressed in BHK cells. Cells were treated as indicated. Top row shows acceptor channel images, and bottom row FRET images. The look-up table shows the FRET-index FR, color coded with high FRET levels in black and yellow (value 1) indicating no FRET. Scale bar is representative for all images and corresponds to 10 µm. (C) Dose-response curves of the effect of DDD85646 on the Emax values of Yes- and Src-NANOMS expressed in BHK cells (n = 6).