Hypo- and Hyperglycemia Impair Endothelial Cell Actin Alignment and Nitric Oxide Synthase Activation in Response to Shear Stress
Figure 5
PKC blockade restored FAK phosphorylation and actin alignment in response to shear stress in cells cultured in high glucose.
A) p-FAK (magenta) and nuclei (blue) with 2 hours Fim-1 diacetate (200 nM) followed by 30 seconds shear stress (horizontal direction). Scale bar = 50 µm. C) p-FAK mean fluorescence intensity. * p<0.01 compared to static. B) Actin (red) and nuclei (blue) for high glucose cells with PKC blocked by 200nM Fim-1 diacetate and 200 nM chelerythrine, and normal glucose cells with PKC activated by 1 µM PMA. Shear stress was applied for 12 hours (horizontal direction). Scale bar = 50 µm. D) and E) Actin aligned fiber percentage and average angle absolute value. * p<0.01 compared to static sample for the same culture condition. Experiments were completed in duplicate and repeated three times.