Leprdb/db Mice with Senescence Marker Protein-30 Knockout (Leprdb/dbSmp30Y/−) Exhibit Increases in Small Dense-LDL and Severe Fatty Liver Despite Being Fed a Standard Diet

doi:10.1371/journal.pone.0065698

Lepr^db/db Mice with Senescence Marker Protein-30 Knockout (Lepr^db/dbSmp30^Y/−) Exhibit Increases in Small Dense-LDL and Severe Fatty Liver Despite Being Fed a Standard Diet

Figure 5

Increased steatosis, inflammation and oxidative stress in liver sections from Lepr^db/dbSmp30^Y/− mice.

Representative images of (A) hematoxylin/eosin staining and (B) 4-HNE immunostaining in liver sections from Lepr^db/+Smp30^Y/+, Lepr^db/+Smp30^Y/−, Lepr^db/dbSmp30^Y/+ and Lepr^db/dbSmp30^Y/− mice. Scale bar is 100 µm. ^*P<0.05, ^†P<0.01 and ^§P<0.001 versus Lepr^db/+Smp30^Y/+, ^#P<0.05, ^‡P<0.01 and ^¶P<0.001 versus Lepr^db/+Smp30^Y/−, ^**P<0.05 versus Lepr^db/dbSmp30^Y/+. Values are given as mean ± SEM of five animals.

doi: https://doi.org/10.1371/journal.pone.0065698.g005