Intersection of Small RNA Pathways in Arabidopsis thaliana Sub-Nuclear Domains
Figure 7
Effect of Cajal body mutants in the localization of miRNA and siRNA pathway components.
Immunofluorescence of several proteins functioning in siRNA and miRNA pathways using specific native antibodies showed that the loss of function of coilin and smD3 interfered with their nuclear localization. Representative nuclei for each class are displayed. In the table, n = number of nuclei analyzed and % indicates the percentage of nuclei with the representative immunolocalization pattern. Nuclear DNA was counterstained by DAPI (in blue). Scale bar denotes 5 µm.