Intersection of Small RNA Pathways in Arabidopsis thaliana Sub-Nuclear Domains
Figure 1
Nuclear localization of trans-acting siRNA pathway members.
Double immunolocalization in interphase nuclei of RDR6, SGS3, DCL4 and AGO7 proteins indicate that, with the exception of RDR6, all the proteins are colocalized within a round-shaped signal in the nucleolar periphery (in yellow in the merged panels). In the top nuclei, RDR6 was detected with a mouse anti-Flag antibody, while SGS3 was detected with an antibody raised in rabbit against the native protein. In the middle and bottom panels, SGS3 was detected using a mouse anti-Flag antibody, whereas DCL4 and AGO7 were visualized by making use of specific native antibodies raised in rabbit. The arrow denotes colocalization foci between RDR6 and SGS3. n = number of nuclei analyzed and % indicates the percentage of nuclei with the representative immunolocalization pattern. Nuclear DNA was counterstained by DAPI (in blue). Scale bar denotes 5 µm.