Class 3 Semaphorin Mediates Dendrite Growth in Adult Newborn Neurons through Cdk5/FAK Pathway
Figure 5
Semaphorin 3A/3F increased dendritic growth in primary neurons and induce tyrosine and serine phosphorylation of FAK.
(A) Representative images showing neurons treated with AP-control (AP), Sema 3A-AP (3A) and 3F-AP (3F). Scale bar = 20 µm. (B, C and D) Graphs showing total dendritic length (B), total number of dendritic branches (C) and axon length (D) of primary hippocampal neurons treated with AP control, Sema3A or Sema 3F. (E, F and G) Representative blots showing phosphorylation of FAK in primary neurons (E and G) and 293T cells (F) treated with AP control, Sema3A or 3F. Graph displays change relative to total FAK and values are mean ± s.e.m, n = 3 independent experiments. 40–50 neurons from each group were used for analysis. Values represent mean ± s.e.m. (**P<0.01, 1-way ANOVA with Newman-Keuls’ post-hoc test).