Synergism between Hedgehog-GLI and EGFR Signaling in Hedgehog-Responsive Human Medulloblastoma Cells Induces Downregulation of Canonical Hedgehog-Target Genes and Stabilized Expression of GLI1
Figure 5
EGF stimulation of Hedgehog-primed Daoy cells strongly upregulated canonical EGF target genes.
Cells were treated with either control medium (purple), Shh-N conditioned medium (blue), EGF (red) or a combinatorial treatment with Shh-N medium and EGF (green). (A) Gene expression profiling data indicated upregulation of MMP7, VEGFA, and IL-8. (B) Taqman Real Time PCR confirmed upregulation of canonical EGF targets in Hedgehog-primed cells. (C) MMP7, VEGFA, and IL-8 protein levels were quantified by ELISA in cell culture supernatants at indicated time points. Average fold-changes or concentrations were obtained as mean of three independent biological experiments (+/−SEM).