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High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD GoldyTALEN Design

Figure 6

Somatic efficacy of 15-RVD TALEN compared with other reported TALEN scaffolds, ZFN and CRISPR/Cas9.

Plot of somatic mutation rates in zebrafish genome editing using ZFNs [8], [32], TALENs [25], [29], [30], [32] and CRISPR/Cas9 [46] from representative publications together with 15-RVD TALENs from this study. *Only Homo-dimeric TALENs were compared. Only 15-RVD TALENs with spacer lengths between 13–20 bp and a T nucleotide preceding the TALEN binding site were included.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0065259.g006