High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD GoldyTALEN Design
Figure 2
Synthesis of 15-RVD GoldyTALENs.
A schematic diagram showing the assembly of 15-RVD GoldyTALENs by the Golden Gate method described earlier [17]. The RCIscript-GoldyTALEN backbone, all other component plasmids (as Golden Gate TALEN kit 2.0), and the 256 pFUS_B4 clones are distributed through Addgene. *XX denoted either NI, HD, NN or NG.