Engineered Monoclonal Antibody with Novel Antigen-Sweeping Activity In Vivo
Figure 5
Effect of sweeping antibody on high plasma concentration antigen.
Effect of NPH-IgG1, PH-IgG1 and PH-v6 on a hFcRn-Tgm steady-state model with high hsIL-6R concentration of approximately 250 ng/mL in the presence of human IgG. NPH-IgG1, PH-IgG1 and PH-v6 were intravenously administered as multiple doses of 0.01 mg/kg every other day. Molar baseline hsIL-6R concentration (6.6 nM) is 5-fold higher than antibody concentration at 15 min (1.3 nM). Time profiles of total hsIL-6R plasma concentration (A) and free hsIL-6R percentage over control (B) are shown. Each data point represents the mean ± s.d. for total hsIL-6R concentration (n = 3–5 each). Free hsIL-6R percentage over control is determined from the pooled plasma sample of n = 3–5 each.