Generation of mRx-Cre Transgenic Mouse Line for Efficient Conditional Gene Deletion in Early Retinal Progenitors
Figure 1
Schematic representation of Cre recombinase-expressing transgenic mouse lines used in this study.
(A) The Rx-Cre; with a 4-kb DNA fragment upstream of the medaka Rx3 gene driving Cre expression [12]. (B) To generate MB31-Cre, the 4-kb DNA fragment upstream of the medaka Rx3 gene was linked to the coding region of Cre recombinase. The IRES sequence was used to connect Cre expression with EGFP to monitor the transgene expression. (C) To generate mRx-Cre BAC, a BAC containing 200 kb covering the Rx locus was modified by BAC recombineering. The Cre coding region (Cre-pA) was inserted into the Rx translational initiation start site (ATG). The exons are indicated with black boxes.