Rosiglitazone Treatment of Type 2 Diabetic db/db Mice Attenuates Urinary Albumin and Angiotensin Converting Enzyme 2 Excretion
Figure 6
ACE2 and ACE activity in urine, plasma and kidney of control, db/db and db/db+rosiglitazone mice using a fluorometric enzyme assay.
(A) Urinary ACE2 activity in control, db/db and db/db+rosiglitazone mice before and after the commencement of treatment. Two-way ANOVA showed an increase in urinary ACE2 activity of db/db mice compared to control mice. Four and eight weeks after treatment commenced there was a significant decrease in urinary ACE2 activity of the db/db+rosiglitazone mice compared to untreated db/db mice. *p<0.001 Vs control mice. #p<0.001 Vs untreated db/db mice. Each bar represents mean ± SEM of group size (n = 6–7). (B) Plasma and renal ACE2 activity in control, db/db and db/db+rosiglitazone mice. There was no plasma ACE2 activity in control and db/db mice but a significant increase in renal ACE2 activity of db/db mice compared to control mice was observed. Treatment with rosiglitazone had no significant effect on renal ACE2 activity of treated db/db mice compared to untreated db/db mice.*p<0.05 Vs control kidney. Each bar represents mean ± SEM of group size (n = 5–8). (C) Plasma ACE activity in control, db/db and db/db+rosiglitazone mice 8 wks after the commencement of treatment. One-way ANOVA showed an increase in plasma ACE activity of db/db mice compared to control mice. Eight weeks after treatment commenced there was a significant decrease in plasma ACE activity of the db/db+rosiglitazone mice compared to untreated db/db mice. *p<0.05, **p<0.001 Vs control mice. #p<0.05 Vs untreated db/db mice. Each bar represents mean ± SEM of group size (n = 6–7).