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Rosiglitazone Treatment of Type 2 Diabetic db/db Mice Attenuates Urinary Albumin and Angiotensin Converting Enzyme 2 Excretion

Figure 3

Histological analysis using PAS and Masson’s Trichrome stain after 8 weeks of treatment with rosiglitazone.

(A) Representative photomicrographs depicting PAS staining of kidney sections in control, untreated and rosiglitazone treated db/db mice. Mesangial matrix and glomerular surface areas were significantly increased in db/db mice compared to control mice. Eight weeks after treatment with rosiglitazone there was a significant decrease in relative mesangial matrix area as well as glomerular surface area compared to untreated db/db mice. *p<0.001 Vs control mice. #p<0.001 Vs untreated db/db mice. Each bar represents mean ± SEM of group size (n = 6–12). (B) Representative photomicrographs depicting Masson’s Trichrome staining of kidney sections in control, untreated and rosiglitazone treated db/db mice. Glomerular fibrotic areas were significantly increased in db/db mice compared to control mice. Eight weeks after treatment with rosiglitazone there was a significant decrease in glomerular fibrotic area compared to untreated db/db mice. **p<0.0001 Vs control mice. Each bar represents mean ± SEM of group size (n = 10–11).

Figure 3

doi: https://doi.org/10.1371/journal.pone.0062833.g003