Antithrombin Regulates Matriptase Activity Involved in Plasmin Generation, Syndecan Shedding, and HGF Activation in Keratinocytes
Figure 4
The shedding of active matriptase is inversely correlated with the levels of membrane-bound AT.
A. HaCaT cells were incubated with the three AT preparations, as indicated, at 37°C for 5 min. The cellular retention of AT were analyzed by immunoblot analyses using an AT antibody. B. The AT-pretreated cells were then induced to activate matriptase by a pH 6.0 buffer. The cell lysates and the conditioned buffers were analyzed for matriptase species and AT species, as indicated. C. Matriptase activity in the conditioned buffer was also assessed by the cleavage of Boc-Gln-Ala-Arg-AMC. Data was done in triplicate and is a representative example of three independent experiments.