Estrogen-Dependent Dynamic Profile of eNOS-DNA Associations in Prostate Cancer
Figure 3
Schematic representation of the miR-34a genomic region and eNOS-peaks identified by ChIP-sequencing.
A) Integrated Genome Viewer (IGV 2.1) screenshots showing pri-miR-34a genomic region (TSS is indicated) and eNOS-binding in C27IM, in the presence or absence of estradiol (E2), identified by ChIP-Seq using two different algorithms MACS and SICER. B) Molecular dissection of orange dashed area indicated in A. Locations of EREs identified by MatInspector analysis are shown by green circles, eNOS peaks identified by MACS are shown by boxes (blue C27IM_NT, red C27IM_E2) and primers for ChIP-qPCR by arrows. C) ChIP validation of eNOS peaks and dynamic recruitment of eNOS and ERβ in the regulatory region of pri-miR34a, in the presence or absence of E2. *p<0,05 ERβ E2 vs ERβ NT; #p<0,05 eNOS E2 vs eNOS NT.