Enzyme Inhibitor Studies Reveal Complex Control of Methyl-D-Erythritol 4-Phosphate (MEP) Pathway Enzyme Expression in Catharanthus roseus
Figure 6
Time course of in vivo fosmidomycin treatment on the expression of MEP pathway genes in young leaves of 6-week-old C. roseus plants at protein and transcript level, respectively.
For each plant, two pairs of mature leaves were injected with a 50 µM fosmidomycin solution. Application procedure and time course of sampling was as described for clomazone treatment (see Figure 5). (A) DXS, DXR and HDS proteins were detected by immunoblot. Equal sample loading was confirmed by Coomassie staining. The arrow marks the position of mature CrDXS2A protein. (B), transcript amounts for DXS isoforms (1, 2A & 2B), DXR, and HDS were determined by qPCR, relative to the geometric mean of multiple reference genes according to Vandesompele et al. [60]. The experiment was performed 3 times, values from a representative experiment are presented ± SD.