Enzyme Inhibitor Studies Reveal Complex Control of Methyl-D-Erythritol 4-Phosphate (MEP) Pathway Enzyme Expression in Catharanthus roseus
Figure 2
Tissue-specific expression of MEP pathway genes in young leaves, mature leaves and roots of 6-week-old non-flowering C. roseus plants, at protein and transcript level, respectively.
Soil-grown periwinkle (Catharanthus roseus) plants were cultivated in a growth chamber at 25°C with 14 hrs light period (170 µmol m−2 s−1) and 22°C during dark period. (A) DXS, DXR, and HDS proteins were detected by immunoblot with polyclonal antisera raised against recombinant CrDXS2A, DXR and HDS proteins. Equal sample loading was confirmed by Coomassie staining. The arrow marks the position of mature CrDXS2A protein. (B) transcript amounts for DXS isoforms (1, 2A & 2B), DXR, and HDS were determined by qPCR (the experiment was performed 3 times, values from a representative experiment are presented ± SD), relative to the geometric mean of multiple reference genes according to Vandesompele et al. [60].