Metabolism of 2-Chloro-4-Nitroaniline via Novel Aerobic Degradation Pathway by Rhodococcus sp. Strain MB-P1
Figure 3
Representative HPLC and GC-MS chromtograms along with mass spectra of metabolites identified during the degradation of 2-C-4-NA by resting cells of Rhodococcus sp. strain MB-P1.
(A) HPLC chromatograms of metabolites identified at different time intervals. Peak at Rt value of 8.5 min corresponds to 2-C-4-NA; Peak at Rt value of 4.86 min corresponds to 4-A-3-CP; Peak at Rt value of 3.28 min corresponds to 6-CHQ. (B) GC-MS chromatogram of the authentic standards and the identified metabolites. Peak at Rt value of 6.41 min corresponds to 2-C-4-NA; Peak at Rt value of 7.62 min corresponds to 4-A-3-CP; Peak at Rt value of 9.52 min corresponds to 6-CHQ. (C) Mass spectra of metabolites (Left side; 4-A-3-CP and right side; 6-CHQ) of 2-C-4-NA as analyzed by GC-MS.