Vitellogenins Are New High Molecular Weight Components and Allergens (Api m 12 and Ves v 6) of Apis mellifera and Vespula vulgaris Venom
Figure 4
Recombinant expression and immunoreactivity of Api m 12 and Ves v 6.
A, B SDS-PAGE and immunoblot analyses of Api m 12 and Ves v 6 recombinantly produced in Sf9 insect cells visualized by Coomassie blue staining, monoclonal anti-V5 epitope antibody and Galanthus nivalis agglutinin (GNA), recognizing terminal mannose 1,2-, 1,3-, and 1,6-linked to mannose. C, D Immunoreactivity of recombinant Api m 12 and Ves v 6 in ELISA using the monoclonal anti-V5 epitope antibody and polyclonal anti-HRP antiserum specific for α1,3-fucose residues, the underlying principle of hymenoptera venom cross-reactive carbohydrate determinant (CCD) reactivity.