In-Situ Determination of the Mechanical Properties of Gliding or Non-Motile Bacteria by Atomic Force Microscopy under Physiological Conditions without Immobilization
Figure 10
AFM height images of the gliding Nostoc (sequence #2).
- Figure 10.a–c: Three AFM height images of the gliding Nostoc bacterium upon the glass slide. In figure 10.c. the bacteria glided away from the AFM scan zone. A common color scale for height was applied for all these images except for the lower part (below the thick white line) of picture (d) where height contrast was enhanced. - Figure 10.d: Visualization of the vertical gliding movement of the Nostoc. It is done by the superposition of the image of the bacteria as determined in figure 10.b (bacterium at the right side of image 10.d) with that measured at t1, 79 s earlier (figure 10.a) and vertically shifted along the white arrow (shift length: 19.3±0.2 microns). For reasons of clarity a lateral shift between the native figures 10.a and 10.b was applied.