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In-Situ Determination of the Mechanical Properties of Gliding or Non-Motile Bacteria by Atomic Force Microscopy under Physiological Conditions without Immobilization

Figure 9

AFM height and stiffness data for Nostoc (sequence #1).

AFM height (a, c) and stiffness (b, d) images of Nostoc in BG11 medium are shown. They correspond to image 7.3. In images (b) and (d), the contrasts were increased to show the presence of the slime layer. Height (e) and stiffness (g) profiles along the black dash/dot line in figures (a–d) are plotted. Height (f) and stiffness (h) profiles along the red dashed line in figures (a–d) are plotted. Stiffness histogram related to the whole stiffness image (figure 9.b or 9.d) is shown in figure (i). In figure (j), the histogram was calculated to the only portion of image 9.b (or 9.d) at the left side of the dotted line. The peaks resulting from the deconvolution of this histogram are plotted with colored lines.

Figure 9

doi: https://doi.org/10.1371/journal.pone.0061663.g009