The Drosophila Microtubule-Associated Protein Mars Stabilizes Mitotic Spindles by Crosslinking Microtubules through Its N-Terminal Region
Figure 5
Overexpression of GFP-N-Mars causes reduced localization of endogenous Mars in the nucleus and on the mitotic spindle.
(A) Wild type embryos and embryos overexpressing GFP-N-Mars were fixed and stained by GFP antibody, Mars antibody raised against the C-terminus (red) and DAPI (turquoise). (B) Wild type embryos and embryos overexpressing GFP-C-Mars were fixed and stained by GFP antibody, Mars antibody raised against the N-terminus (red) and DAPI (turquoise). GFP channels not shown. Scale bar is 5 µm.