DOTA-Functionalized Polylysine: A High Number of DOTA Chelates Positively Influences the Biodistribution of Enzymatic Conjugated Anti-Tumor Antibody chCE7agl
Figure 3
Enzymatic conjugation of (DOTA)n-decalysine to chCE7degl or chCE7agl, an aglycosylated variant of the antibody.
Although there are two enzymatic recognition sites per heavy chain of chCE7agl (Gln295 and Gln297), only one molecule of (DOTA)n-decalysine (n = 1, 3, 5) was conjugated using transglutaminase. Interestingly, two isopeptide bonds were formed between one substrate and one heavy chain, as indicated by the loss of two ammonia molecules (bottom).