Antifungal Efficacy during Candida krusei Infection in Non-Conventional Models Correlates with the Yeast In Vitro Susceptibility Profile
Figure 10
Virulence of C. krusei and C. albicans in C. elegans and antifungal efficacy.
Caenorhabditis elegans was infected as described in material and methods with C. krusei (ATCC 6258), C. albicans (SC5314) and E. coli OP50. (A) Visual appearance of infected worms (50× magnification). (B) Antifungal efficacy in C. elegans infected with C. albicans. ♦ OP50, • C. albicans, ▪ C. albicans and treated with 2 µg/mL amphotericin B (p<0.0001), ▴ Fluconazole 12 µg/mL (p<0.0001); ▾Caspofungin 2 µg/mL (p<0.0001). (C) Antifungal efficacy during C. krusei infection ♦ OP50; • C. krusei; ▪ C. krusei treated with amphotericin B 2 µg/mL; (p<0.0001); ▴Fluconazole 12 µg/mL (p = 0.1207); ▾Caspofungin 2 µg/mL (p<0.0001). (D) Effect of voriconazole on survival of C. elegans worms infected with C. albicans (•, C. albicans, ▴, voriconazole 0.25 mg/L (p<0.0001); ▪, voriconazole, 7.5 mg/L (p<0.0001); ▾ voriconazole 10 mg/L (p<0.0001)). (E), Efficacy of voriconazole during infection of C. elegans by C. krusei (•C. krusei; ▴voriconazole 0.25 mg/L (p = 0.1217); ▾ voriconazole 7.5 mg/L (9<0.0001); ▪ voriconazole 10 mg/L (p<0.0001)).