Nicaraven Attenuates Radiation-Induced Injury in Hematopoietic Stem/Progenitor Cells in Mice
Figure 5
Intracellular and mitochondrial ROS in bone marrow cells.
Mice were exposed daily to 1 Gy γ-rays for 5 days in succession, and either nicaraven or a placebo was given after each exposure. The cells were collected 2 days after the last radiation treatment and then loaded with 10 µM CM-H2DCFDA or 5 µM MitoSOX Red at 37°C for 30 minutes. The intracellular ROS (A) and mitochondrial ROS (B) were detected as the mean fluorescence intensity in all cells by flow cytometry. The open circles represent data from each mouse and the red lines indicate median values of each group.