Lavender Oil-Potent Anxiolytic Properties via Modulating Voltage Dependent Calcium Channels
Figure 5
Silexan inhibits N- and P/Q-type VOCCs in whole cell patch-clamp experiments.
(A) Whole cell recordings of N-type VOCCs were conducted in CHO cells stably expressing N-type Ca2+ channels. Silexan (1 or 10 µg/ml) was applied by superfusion for 50 s. The cells were stimulated with a depolarizing pulse to 20 mV at 1 Hz and the amplitude was recorded (n = 9–14). (C, D) Representative currents of whole cell patch clamp recordings with Silexan 1 and 10 µg/ml in N-type cells when stimulated with a depolarizing pulse to 20 mV before (ctl) and at the end of Silexan application (+ Silexan). (B) Effect of preincubation of Silexan (1–10 µM) on depolarizing pulses to 20 mV in P/Q-type cells (n = 4–5). (E and F) Time course of the N-type Ca2+ channel peak current amplitude for 125 seconds by depolarizing pulses to +20 mV at a pulse frequency of 1 Hz. Inhibition of the N-type Ca2+ channel peak current amplitude by Silexan 1 µg/ml (E) or 10 µg/ml (F) compared to DMSO. The arrows indicate the time points of the Silexan application and the washout. A total number of 10 (DMSO), 14 (Silexan 1 µg/ml) and 9 cells (Silexan 10 µg/ml) were averaged. All data presented are mean values ± SEM (unpaired t-test).