Remodeling of Oxidative Energy Metabolism by Galactose Improves Glucose Handling and Metabolic Switching in Human Skeletal Muscle Cells
Figure 5
Effect of galactose treatment on mitochondrial and neutral lipid content.
Myotubes were grown in DMEM-media with 5.5 mM glucose or 5.5 mM galactose. The cells were stained for mitochondria, neutral lipid and nuclei as described in Materials and Methods. The figures show (A) pictures of stained myotubes with mitochondria (red), neutral lipids (green) and nuclei (blue), (B) mitochondrial content, (C) neutral lipid content. Results represent fold change relative to glucose given as means ± SEM, (n = 3) and the data are normalized to number of nuclei. *P<0.05 vs. glucose. Arbitrary units for mitochondrial content per nucleus and neutral lipid content were 552232±137107, 860881±218244, respectively for glucose and 942449±71302, 1299938±102390, respectively for galactose.