2,2′-Diphenyl-3,3′-Diindolylmethane: A Potent Compound Induces Apoptosis in Breast Cancer Cells by Inhibiting EGFR Pathway
Figure 4
Activation of mitochondrial caspases and induction of apoptosis in DPDIM treated breast cancer cells.
(A), Activation of mitochondrial caspases-9, 7 and 3 in 24 hr DPDIM treated cells were shown by IB. (B), Analysis of PARP cleavage was done by IB in MCF7 and MDA-MB 231 cells after 24 hr treatment with DPDIM. (C), Apoptotic cell population was evaluated after 24 hr of treatment by FACS analysis using double staining with Annexin V and PI. (D), In situ TUNEL assay showing inter-nucleosomal degradation of genomic DNA in 48 hr treated MCF7 cells. Cells were stained with DAB and counterstained with methylgreen. The % TUNEL positive cells were calculated and the quantitative evaluation represented in the bar diagram with SD. *indicates P<0.0001. All results are representative of three independent experiments.