Levels of Lycopene β-Cyclase 1 Modulate Carotenoid Gene Expression and Accumulation in Daucus carota
Figure 5
Over expression of DcLcyb1 increases carotenoid and β-carotene levels and expression of key carotenogenic genes.
Relative expression of DcLcyb1 gene in L6, L8 and L9 from (A) leaves and (B) storage roots of transgenic carrot plants. Ubiquitin was used as normalizer in qRT measurements. Carotenoids of D. carota L6, L8 and L9 transgenic lines were extracted from (C) leaves and (D) storage roots and carotenoid composition was determined by spectrophotometry and High Performance Liquid Chromatography. Relative expression of DcPsy1, DcPsy2 and DcLcyb2 in transgenic carrot lines from (E) leaves and (F) storage roots. Ubiquitin was used as normalizer in qRT measurements. For gene expression and carotenoid analysis three months-old plants were used. Columns and bars represent the means and SE (n = 3). Asterisks indicate significant differences between transgenic lines and the wild type plant. Non-paired one and two tailed t-tests (p<0.05) were performed for all the transgenic lines and the wild type plant.