Effectiveness of Annealing Blocking Primers versus Restriction Enzymes for Characterization of Generalist Diets: Unexpected Prey Revealed in the Gut Contents of Two Coral Reef Fish Species
Figure 2
Variability at each position of the COI 3′ end region for the design of annealing blocking primers.
Entropy plots were performed using 947 decapod COI sequences (A) (≈330 species), 758 fish COI sequences (B) (≈380 species) and 271 gastropod COI sequences (C) (≈170 species) collected and sequenced by the Moorea BIOCODE project. The black line shows the position where predator specific annealing blocking primers were designed for the two hawkfish species in order to minimize the probability of blocking prey amplification. The black box displays a region of high sequence variability across taxa making it a good-quality binding site for the 3′end of predator specific annealing blocking primers.