Tissue Engineered Skin Substitutes Created by Laser-Assisted Bioprinting Form Skin-Like Structures in the Dorsal Skin Fold Chamber in Mice
Figure 5
Blood vessel detection via immunohistochemistry in skin constructs cultivated in vivo for 11 days.
Skin constructs were cultivated in vivo for 11 days in the dorsal skin fold chamber in mice. Collagen IV expression (brown) – indicating blood vessels/capillaries – can be detected in the Matriderm® as small tubes reaching from the wound bed in the direction of the cells (A). Small and large blood vessels are present in the normal mouse skin (B). Matriderm® (C) and normal mouse skin (D) without first antibody serve as the respective negative controls. Scale bars depict 200 µm each.