Altered Reward Circuitry in the Norepinephrine Transporter Knockout Mouse
Figure 5
Histology of the injection site showing precise location and minimal damage.
Left panel: A representative example of one brain section through the forebrain stained with Solochrome for both nuclei and fiber tracks and imaged at low magnification shows the injection site in the right frontal cortex (boxed region). Although the co-injected oil droplet identifying the injection site, leaves a small displacement, there is no bleeding, gliosis, or other indication of injury. Scale bar = 200 μm. Right panel: Fluorescent micrograph of an adjacent section of the same brain. The injection site is detected by fluorescent signal from the co-injected rhodamine-dextran tracer (red). Note that the tracer is confined to the region of the oil droplet and a few microns above it. Some auto-fluorescence of red blood cells within parenchymal capillaries is scattered in the background. Anatomical features revealed by a counter-stain for cellular nuclei with DAPI (blue) confirm that the location is within the forebrain. Scale bar = 20 μm.