Characterization of Ascites-Derived Ovarian Tumor Cells from Spontaneously Occurring Ovarian Tumors of the Chicken: Evidence for E-Cadherin Upregulation
Figure 5
Migration and wound healing properties of chicken ovarian cancer (COVCAR) cells.
A vertical wound (700–900 µm width) was created in fully-confluent COVCAR cells followed by washing in culture medium and incubation at 37°C and 5% CO2. Photomicrographs were taken at 0, 8, 16 and 24 h post-wound creation to determine the wound area using image analysis software. A–D. Representative photomicrographs of the wound at 0, 8, 16, and 24 h after creating wound in C11 cell line in 4th passage. E–H. Wound area following 0, 8, 16, and 24 h in COVCAR cell lines C5 (E), C6 (F), C11 (G), and C19 (H). Data are represented as mean ± standard error of the mean from 3 replicates., P≤0.0001 COVCAR vs NOSE.