Dissemination and Transmission of the E1-226V Variant of Chikungunya Virus in Aedes albopictus Are Controlled at the Midgut Barrier Level
Figure 2
Effect of E1-A226V substitution on CHIKV infection in Ae. aegypti (AAPT) and Ae. albopictus (ALPROV).
Ae. aegypti (light-grey) and Ae. albopictus (dark-grey) were orally infected with a blood-meal containing both E1-226A and E1-226V provided at the same titer, 106.5 pfu/mL. Every day, 5 mosquitoes were sacrificed to isolate the midgut. The proportion of E1-226V compared to E1-226A (A) and the number of infectious particles (B) were determined. Error bars show the confidence intervals (95%) for % CHIKV E1-226V and the standard deviations for Log10 pfu/midgut.