The Ciliary Protein Ftm Is Required for Ventricular Wall and Septal Development
Figure 5
Shh and Pdgfrα signals are downregulated in Ftm-negative murine hearts.
(A–D) Real-time PCR analysis of wild-type and Ftm-deficient ventricular (A, B) and atrial tissue (C, D) at E11.5 (A, C) and E14.5 (B, D). (A) Shh target gene expression of Ptc1 and Pdgfrα target gene expression of Hif1α are significantly downregulated in E11.5 Ftm−/− ventricles (n = 3, respectively; Ptc1: p = 0.013; Hif1α: p = 0.005). (B) At E14.5, both signaling pathways are unaffected in Ftm-negative ventricles (n = 6, respectively). (C, D) In Ftm−/− atria, Shh and Pdgfrα signaling are not significantly altered at E11.5 (n = 6 atria, respectively; C) and at E14.5 (n = 6 atria, respectively; D).