β-catenin Is Essential for Efficient In Vitro Premyogenic Mesoderm Formation but Can Be Partially Compensated by Retinoic Acid Signalling
Figure 5
RA can recover the expression of most skeletal muscle precursor genes in P19[shβ-cat] cells.
Aggregated P19[shControl] and P19[shβ-cat] cells were differentiated in the presence of 1% DMSO with 3 nM RA. Panels I & II: RNA was harvested on days 0, 5 and 9 of the differentiation and analyzed by QPCR for all days (Panel I) or days 0 & 9 (Panel II). Changes in gene expression were normalized against β-actin represent fold change over day 0 levels in P19[shControl] cells. Error bars correspond to the ± SEM. Panel III: Cultures were fixed on day 9 for immunofluorescence with an anti-MHC antibody. The number of MHC+ve cells were counted and expressed as a percentage of the control cells. Error bars represent ± SEM (n = 6). The Student’s t-test was used to assess statistical significance, where *p-value ≤0.05.