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Identification and Characterization of Cells with Cancer Stem Cell Properties in Human Primary Lung Cancer Cell Lines

Figure 5

Flow cytometry analysis of CD44 and CD90 expression in the PLCCLs.

Staining by anti-CD44 FITC and anti-CD90 APC. All the cell lines showed heterogeneous staining and could be divided into 4 sub-populations: CD44^highCD90⁺, CD44^highCD90⁻, CD44^low/−CD90⁺ and CD44^low/−CD90⁻. For the SCLC cell line LC004 (A), the frequency of CD44^highCD90⁺ cell was 16.6%, and the CD44^highCD90⁻ cells was 8.2%. For the LCC cells line LC006 (B), the frequency of CD44^highCD90⁺ cells was 1.1%, the CD44^highCD90⁻ cells was 2.5%. For the AC cell line LC007 (C), the frequency of CD44^highCD90⁺ cells was 9.4%, and the CD44^highCD90⁻ cells was 23.4%. For the SCC cell line LC021 (D), the frequency of CD44^highCD90⁺ cells was 2.3%, the CD44^highCD90⁻ cells was 1.1%.

doi: https://doi.org/10.1371/journal.pone.0057020.g005